Nokuti chinangwa mharidzo iri rakaitirwa Coombs?

Coombs - chaiyo rabhoritari kuongororwa kuti unobvumira kuona antibodies iri yeplasma kana matsvuku eropa pemvura. nzira iyi kunoita rokuongorora zvirwere hemolytic kupererwa, kusanganisira achangoberekwa, uyewo kuziva hemolytic ropa zvavaifunga. Coombs ari kushingaira kushandiswa pakutsvakurudza forensic sayenzi genetics kuziva erythrocyte maantigen. Zvaidiwa pemitemo yose yakadaro kuongorora runoita kuti chakavimbika kupfuura zvabuda.

Chinangwa antiglobulin bvunzo

Direct Coombs Muedzo kuziva Anti-erythrocytic antibodies, izvo pazvinhu erythrocytes. Positive paakasangana yakafanana kudzidza rinotaura kuti pave autoimmune hemolytic kupererwa neropa. Zvinofanira kucherechedzwa kuti zvakaipa mugumisiro hakurevi haabatanidzi kuvapo autoimmune chirwere, sezvo antibodies kazhinji kuwanikwa vakasununguka chimiro, i.e. havana chokuita erythrocytes. Muzviitiko zvakadaro zvinokurudzirwa kuti aite zvisina kunanga Coombs bvunzo, iyo achasarudza vanhu zvionera nevamiririri mu serum.

Sei kuongorora?

W Mwanda venous ropa murwere rinoitwa usina chawadya mangwanani, pasinei nokuti chero zvingawedzera kuti kupesvedzera wokupedzisira mugumisiro yokuedzwa ichi, haina kuratidzwa. Store zvinhu huchibvumirwa akatorwa pana 2 kusvika 8 ° C kwete refu kupfuura mazuva manomwe. To zviratidzi chidzidzo ichi vaiva sezvo chaiwo sezvinobvira, ropa rose munguva maawa maviri kutanga agoiswa murabhoritari. Zvakanakisa Coombs mharidzo vanofanira kuratidza munhu akaipa mugumisiro, zvichiratidza kusavapo hemolytic kuchinja pamuviri.

achiongorora zvaive kwadzo

Coombs iri nani rakaoma tsvakurudzo nzira, iyo zvinoda kungwarira uye yakarurama. Kana uchishandisa nemuedzo wakadaro kunogona vasangana zvimwe zvinetso kuti chokuita sizvo kududzirwa okupedzisira zvabuda nokuda kuratidzwa simba rekuva siyana. Zvinofanira kucherechedzwa kuti unreliability ongororo - kureva zvakanaka Coombs - angava migumisiro va neudhadha Washing erythrocytes kuonana nuna
nomumativi neutralization zvinoriumba antiglobulin reagents serum. Chimwe zvakaipira nzira iyi kusagadzikana kudzidza atora zvinhu rakachengetedzwa ine zvimwe zvinhu.

The kukonzera venhema negative zvinogona kunyanyisa vachidzungudza kuturika kuti erythrocytes mu resuspension. Dzenhema nemigumisiro zvingakonzerwawo nokuda pamberi anticomplement antibody tsvina izvo adsorbed pamusoro pemvura panguva incubation rebvunzo erythrocytes, nokudaro kusika nechitarisiko zvakanaka mugumisiro. Kana vamboshamba bvunzo ivhu uye kuongorora maitiro ezvinhu, payakaipira izvi zvinogona nyore kubviswa, izvo kuchawedzera mikana kuwana chakavimbika kupfuura zviratidzi ose Coombs bvunzo.