PCR ongororo: chii? Sei kutora PCR ongororo

Hazvina kare saka refu yakatanga yakavimbika, zvikuru kugumbuka uye nokukurumidza nzira nokuda nechirwere-siyana zvinotapukira muvanhu. nzira kwakadaro anonzi "PCR ongororo". Chii, chii chacho zvazviri, chii anogona kuziva zvigare uye sei kuti kuitora, tichaita tsanangura munyaya ino.

History yakawanikwa

Akazogadzira polymerase cheni maitiro (PCR) chiri musayendisiti American Keri Myullis muna 1983. Pakutanga, patented nzira nechirwere ne "Cetus Corporation," umo iye aishanda uye ayo musiki. Asi muna 1992 kodzero dzose uye patents vakatengeswa kuti kambani "Hoffman-La Roche." Pashure paizvozvo, ndakaona kuti kudzidza yakafanana rwaiitwa mune zvakafanana uye vakanga kushumwa vamwe US zvipenyu, akadai Elis Chen, David Edgar, John rre. Muna 1980 iye vaita ichi nyaya uye masayendisiti Soviet A. Slyusarenko A. Kaledin uye S. Gorodetsky. Saka, kuona bedzi muridzi Copyright haana zvichikwanisika. kunoshamisa biochemists Vakawanda vakabatsira kuti kukura nzira polymerase kuita uye CERN patented zvakagadzirwa dzavo. Panguva iyi pfungwa kuongorora PCR rinoitwa panyika zvikuru akashongedzerwa mumarabhoritari.

Pfupiso PCR chirwere

PCR ongororo: chii uye sei kushanda? Musimboti nzira iri chokwadi kuti nokushandisa chaiyo enzayemu DNA polymerase ezvokunambira kunze kwomuviri waamai dzichiwedzera vhoriyamu zvakananga microbial ezvinhu. Kuti aite izvi kakawanda muwande iripo DNA zvinhu. Saka, kuvapo utachiona hwakakura ari mharidzo mari achawedzerwa tichiregeredza nokukanganisika rabhoritari kumonyanisa uye utachiona hazvizovi zvakaoma kuona ari maikorosikopu.

Sei kudzidza zvinhu?

Kuti aite ongororo kunodiwa:

• The DNA template;
• primers zvinhu unobatanidza kumagumo chinyorwa;
• thermostable DNA polymerase enzayemu;
• mishonga, nechokwadi ichishanda zvakanaka makemikari;
• buffer mhinduro inodiwa kusika zvakakodzera ezvinhu kuti kukura DNA zvokunyama.

Kuti aite PCR ishande 25-30 anodzokorora rinoumbwa matanho matatu: denaturation, annealing uye elongation.

Kuongorora polimezarnoy cheni maitiro kushandisa zano chairo - cycler. Modern midziyo kunoita iwe kuisa zvakakodzera chirongwa kudziyisa uye kutonhora sepombi kubvisa zvikanganiso panguva nechirwere.

Where kushandisa chirwere?

Polimezarnoy cheni maitiro nzira zvinoshandiswa minda mushonga siyana:

• Forensic kushandiswa kuti kuziva majini zvinhu, zvakadai bvudzi, mate kana ropa;
• PCR Kuongororwa ropa kunobatsira genotyping, semuenzaniso kuti vasabatwa zvemunhu nemagene akadyara norudaviro chairo mushonga;
• achishandisa nzira kuisa ukama hwemhuri pakati vanhu;
• vakawanda vaitsvaka-PCR nzira zvava mukurapwa chirwere nokuda Kutsunga siyana zvinotapukira.

Ndezvipi zvirwere anoratidza PCR?

Saka, mushonga yagara zvinobudirira kushandiswa PCR ongororo. Chii, atova tadzidza. Uye vamwe utachiona vanogona kuonekwa nacho? PCR chirwere anotevera zvirwere :

• chiropa A, B, C;
• ureaplasmosis;
• candidiasis;
• chlamydia;
• mycoplasmosis;
• kwemabhakitiriya vaginosis;
• rwarisa mononucleosis;
• trichomoniasis;
• HPV chirwere;
• tiibhii;
• herpes hweHIV of 1st uye 2nd mhando;
• Helicobacter pylori chirwere;
• cytomegalovirus;
• diphtheria;
• salmonellosis;
• HIV.

Uyewo, PCR nzira inoshandiswa mune chirwere kenza.

zvakanakira nzira

Chirwere PCR ane dzakawanda zvakwakanakira:

1. High utetsa. Kunyange mashoma DNA molecules microorganism PCR ongororo anosarudza kuvapo chirwere. Zvinobatsira nzira kuti chirwere uye Isimba rakadini rinogona kubudiswa zvirwere kuitika. Kazhinji Muzviitiko zvakadaro, microorganism iri uncultivated mune dzimwe nzira.
2. Kuti kuongorora chero yakakodzera zvinhu, zvakadai nemate, ropa, pabonde selection, bvudzi, masero epithelial. Rakajairika ndiyo bvunzo ropa uye urogenital swab PCR.

3. Kwete inoda kuwedzerwa kukura tsika. The zvoga okumugamuchira Diagnostics inobvumira kuwana migumisiro kudzidza pashure maawa 4-5.
4. Nzira iri rinenge chose zvechokwadi. Yakanyorwa chete oga zviitiko dzenhema negative mugumisiro.
5. Kugona kuziva mhando inoverengeka utachiona mune imwe mharidzo zvinhu. Izvi kwete chete kunowedzera chirongwa nechirwere kwechirwere chacho, asiwo yekudyidzana kunoderedza zvinhu mari. Kazhinji, chiremba prescribes misoro Kuongorora PCR. Price kuongorora, zvinosanganisira kuziva utachiona matanhatu, iri pamusoro 1,500 rubles.

Zvinotarisirwa pasi kugadzirira ongororo

Migumisiro kuti ishande PCR ongororo vanofanira kuongororwa, vachitevera kunenge ari rokutanga gadziriro nechirwere:

1. Usati wakurukura kuti nemate haafaniri kudya uye zvinodhaka nokuda 4 maawa asati zvakabudiswa mashoko. Pakarepo pamberi nzira, sukurudza muromo wako yakabikwa nemvura.
2. The pamusoro mitemo kunofanira kutungamirirwa uye kana achitora mharidzo kubva mukati padama. Pashure rinsing inokurudzirwa kuti aite chiedza masaja paganda kusunungura prostatic secretions.
3. Weti kakawanda inounganidzwa kumba. Kuti aite izvi, unofanira kuitisa anonyatsotsvaka chimbuzi kuti sikarudzi. Mune chena epurasitiki mudziyo zvinokosha kuti vaunganidze 50-60 ml weti. It akakurudzira vakadzi kuti dzinoisa mashoko tampon kupinda nyinyo, uye varume napo kudhonza ganda peta inonzi kuchena acho. Haukwanisi kutora mashoko panguva varikutsika.
4. Nokuti chokuendesa dzisingaoneki kurega kurara mumazuva 3 asati muunganidzwa zvokunyama. Uyewo, vanachiremba vari zano kusiya kushandiswa sauna uye inopisa kugeza, doro uye Spicy zvokudya. Kwemaawa 3 risati ongororo zvinokosha kurega vanhurume.
5. Zvakaitika urogenital zodzera Somuenzaniso, kana kuongorora pamusoro chlamydia PCR, vose vakadzi nevarume inokurudzirwa kuti kurara zororo 3 days. 2 mavhiki kuongorora haagoni kutora mishonga inorwisa mabhakitiriya. Kwevhiki unofanira kurega kushandisa rakapfava gels, mafuta okuzora, kusangana nekutsi kwemukadzi suppositories, douching. maawa 3 risati kudzidza haafaniri vanhurume. Panguva yekutevera fenzi zvinhu hazvina kuitwa, mazuva 3 chete pashure kugumiswa kusvibisa anogona kutora urogenital zodzera.

PCR panguva pamuviri

The vakamirira nguva kuti mwana, vazhinji zvirwere, zvirwere zvinopomeranwa pabonde, kune ngozi chaizvo nokuti kwomuzvarirwo fetus. STDs kungamutsawo intrauterine kukura, nokupfupfudzika kana magadzirira kubereka, vachiberekwa malformations mwana. Naizvozvo, zvakakosha kuti in early pregnancy achivhara PCR. Pass ongororo kunokosha kana kunyoresa - kusvikira vhiki 12.

Fence zvinhu rinobva remuromo Canal chaiwo brushes. The nzira iri nyarara uye kunoisawo hapana ngozi mwana. Kazhinji panguva pamuviri vakaongorora nokuda Chlamydia PCR nzira, uyewo ureaplasmosis, mycoplasmosis, cytomegalovirus, herpes, papillomavirus. Kwakadaro kuongororwa yakaoma inonzi PCR 6.

PCR nokuti chirwere HIV

Nokuti kuti nzira polymerase Chain kuita zvikuru kunzwisisa kuchinja mumuviri uye mamiriro ezvinhu nechirwere, zvinhu zvakawanda zvinogona kukanganisa zvaizoitika. Naizvozvo PCR Kuongororwa kuti neHIV haisi nzira yakavimbika, ayo kushanda - 96-98%. The yasara 2-4% of bvunzo anopa nhema yakanaka.

Asi mune mamwe mamiriro ezvinhu pasina PCR chirwere neHIV haagoni kuita. Kazhinji, voitakura kuvanhu kwenhema negative Elisa. Izvi inofananidza anoratidza kuti munhu wacho haasati yakatanga antibodies kuti utachiona uye haagoni kuonekwa pasina kuwedzera vakawanda. Kuti izvi zvinogona kuitwa pakuitisa bvunzo ropa PCR nzira.

Vaifanirawo ndiyo chirwere vana gore upenyu rokutanga, vanoberekwa HIV. Nzira ndiyo chete nzira yakavimbika kuona mamiriro mwana.

PCR nokuti chirwere kuti nehepatitis

Polymerase cheni maitiro nzira anobvumira kuziva miedzo DNA kweutachiwana ose nehepatitis A, B, C, zvakanaka pamberi nokuumbwa antibodies kuti chirwere kana chiratidzo Pazvinotanga. Abudirire ndiye PCR ongororo pamusoro hepatitis C, kubva 85% zvirwere kwakadaro asymptomatic uye pasina nenguva kurapwa anova refu.

Panguva Chaiyo kuonekwa pakati nayo utachiona kuchabatsira kudzivisa matambudziko uye kurapwa kwenguva refu.

Integrated PCR kuongorora

Nzwisisika PCR Analysis: chii? Izvi rinovharidzira nzira polimezarnoy cheni maitiro izvo zvinosanganisira kusarudza panguva dzakawanda mhando chirwere: Mycoplasma genitalium, Mycoplasma hominis, Gardnerella vaginalis, Candida, trichomonas, cytomegalovirus, ureaplasma urealitikum, herpes 1st uye 2nd mhando, mwandabvu papillomavirus. Mutengo nechirwere ichi anoda kubva 2,000 kusvika 3,500 rubles. zvichienderana mukiriniki, zvinhu uye midziyo, uyewo pamusoro mhando ongororo: Qualitative kana quantitative. Chii chinodiwa nyaya yako - chiremba anosarudza. Mune zvimwe zviitiko, iwe chete kungotaura ticherechedze pamberi nayo utachiona vamwe, vakadai HIV, rinokosha basa reDare quantitative titer. kunzi "PCR kuongorora 12" iri nechirwere zvose zviri pamusoro utachiona kuongorora.

Achiongorora zvaive zviri kuongorora zvabuda

Transcription PCR ongororo akananga. Pane 2 chete indekisi pamwero - ". Kunaka" "zvakanaka" uye Pamusoro kuonekwa pakati nayo utachiona vanogona vanachiremba vane 99% chivimbo -th kuzosimbisa pamberi chirwere uye kutanga kurapwa murwere. Mugore quantitative nzira dzokuziva chirwere mune runoenzanirana mbiru indekisi nhamba dzakaonekwa mabhakitiriya zvichabviswa kutaurwa. Chete chiremba anogona kuona kukura kwakaita chirwere uye varayire anodiwa kurapwa.

Mune zvimwe zviitiko, somuenzaniso kuziva HIV ne PCR, ane kunaka mugumisiro, pane kudiwa okuwedzera kuongorora kuzosimbisa nenhamba idzi.

Where kuti kuedzwa?

Apo zvakaitika-PCR kuongorora pachena kukiriniki kana muchihwande rabhoritari? Zvinosuruvarisa, ari matunhu neminisiparati zvokurapa masangano midziyo uye nzira kazhinji yechinyakare. Naizvozvo zviri nani kupa kupfuura voga mumarabhoritari vane midziyo ano uye vashandi zvikuru vanokwanisa. Mukuwedzera, vari voga pakiriniki iwe kuwana nemigumisiro zvikuru nokukurumidza.

MuMoscow, dzakawanda private mumarabhoritari kupa PCR Kuongororwa kuti zvirwere zvakasiyana-siyana. Somuenzaniso, mune makiriniki akadai "Vita", "Integrated Clinic," "Happy Family", "Uro-Pro", kuitwa PCR ongororo. Survey mutengo uri 200 rubles. nokuti Pakusarudza ane utachiona hwakakura.

Zvinogona vakagumisa kuti nechirwere kwezvirwere zvinotapuriranwa kubudikidza PCR kakawanda chinhu nokukurumidza uye yakavimbika nzira Detective nayo utachiona mumuviri panguva tanga chirwere. Asi, mune zvimwe zviitiko, kusarudza dzimwe nzira nechirwere. Kuona kudiwa kudzidza kwakadaro kunogona chete kuva nyanzvi. PCR Kuongororwa Decryption anodawo nyanzvi nzira. Tevedzera zano chiremba wako uye musapa miedzo yavo, izvo zvisina kudiwa.