Alanine aminotransferase yakawedzera - chiratidzo anogumbura uye chinopedza pezvakaitwa vari nhengo

Nokuda aminotransferase dzichitama kubva NH2-mapoka vane γ-amino iine acid anyatsoronga kuti acceptor - α-amino asidhi pasina zvenguva paumbwe Ammonium. Kureva transamination (transamination: reversible kutapurirana mapoka amino kuti keto acid) - mumwe muitiro rakazaruka rakakurumbira Masayendisiti Russian uye Kritsman Braunstein.

Amino acids (aspartic, glutamic uye alanine) panguva transamination mudzoke kunyika unoenderana α-keto acid, izvo zvinoumba nyika tricarboxylic asidhi yatinofema. Panguva oxidation yavo Krebs kutenderera, ivo mune simba.

Transamination kunokosha pakupa mashoko urea kutenderera aspartate.

Pasinei nokuti transamination siyana vanogona pachena uye anonhuhwirira amino acids: phenylalanine, tyrosine uye tryptophan - kushanda mune zvokurapa yakazviwanira tsanangudzo webasa aspartate uye alanine aminotransferase. makemikari aya rinoparadzirwa munyama womunhu muviri.

Alanine aminotransferase yakawedzera masero matsvuku eropa wayo yevasungwa ndiyo katanhatu yakakwirira zvichienzaniswa kuti ropa serum. The chepamusorosoro wevasungwa pamusoro enzayemu ndee mune chiropa munyama.

Alanine aminotransferase ndeyokuva kwete chete nokuti muviri womunhu munyama. Somuenzaniso enzayemu basa iri cardiac zvinyama iri 7.1 (E * 10-3 d rinenge homogenate), chiropa - 44, skeletal tsandanyama - 4,8, itsvo - 19, chirwatata - 1,2, 0,7, uye bapu serum - 0,016. Huripo nzira kuti kutsunga aminotransferase basa iri serum rakakamurwa mapoka maviri: asi endpoint (colorimetric, vachishandisa kwakajairika, kwete akashongedzerwa aine tembiricha kudzorwa cuvette photometric midziyo) uye kinetic (spectrophotometric).

B6 vanowanzochema vhitamini zvakasiyana-siyana zvokudya. Zvisinei, tinofanira kuyeuka kuti panguva yokubikira muitiro (thermal obrobka) ari vhitamini raparadzwa. Pyridoxine ari cofactor mu makemikari akawanda. Wevasungwa pirodoksin-5-phosphate muropa shoma zera. Saka, basa enzayemu uyu alanine aminotransferase, yakaderera vanhu vakura kupfuura makore 64 (anenge 30%) pane makore 46-63 makore iwayo. Wevasungwa vhitamini B6 muropa serum alanine aminotransferase dzenharo.

Kuona dzichipfekeka mamiriro chiropa zvinowanzooneka vaedzwa aminotransferase nhanho muropa zvomurwere. Izvi imhaka zvikurukuru chokwadi kuti anochinja pabasa ravo kunoitika nokukurumidza zvikuru kudarika mamwe rabhoritari parameters. Kuchaitwa (mbudo) chiropa makemikari muropa kunoratidza cytolysis - sero kuparadza kana nokuvhiringidzirwa zvavo Kamudzira permeability. Kana kuongorora kuchinja mubasa makemikari inokosha kumisa nzvimbo kuchaitwa. Unofanira kuziva basa ravo mumasero ropa uye kumuviri, asi subcellular localization uye muviri. The enzayemu wevasungwa - alanine yakawedzera, kunyanya chitaka mumasero, saka, kunyange zvishomanana kukuvara chinokurwadzai dzavo kuwedzera enzayemu basa muropa. Akapiwa basa enzayemu rokuongorora zvirwere chiropa (chiropa, steatosis).

Alanine aminotransferase yakawedzera necrosis chiropa, uyewo parenchymal chiropa. The pasi pfungwa yacho enzayemu rinonyoreswa mune chirwere kuzvimba chiropa uye dystrophy. Zvinofanira akati kuti kuwedzera aminotransferase basa iri serum rinotanga mazuva 3-8 pamberi Pazvinotanga huru zvekuchipatara zviratidzo chirwere uye rinosvika vaifanira mumazuva kukura pathological muitiro mangwanani.

Saka, alanine aminotransferase yakawedzera: cirrhosis chiropa, jaundice, kenza yechiropa, myocardial infarction, kurudyi mwoyo kukundikana, hypoxia, kuvhunduka, myositis, myocarditis, steatosis, pancreatin, chisingaperi chidhakwa.