Zvose pamusoro enzayemu pepsin

Nyaya iyi anotarira hunokosha makemikari kuti varipo mudumbu ose mhuka, kusanganisira vanhu. muchidimbu enzayemu pepsin, mashoko pamusoro isomers ayo, ichakurukurwa uye basa vamiririri iri dzinotsanagura kugaya.

General maonero

Chokutanga, ngationei kuti boka makemikari zvinosanganisira pepsin. Izvi zvichaita kuti pakunzwisisa zvakadzama wenyaya pachayo.

The enzayemu pepsin proteolytic chokuita boka hydrolases anobudiswa uye gastric mucosa, uye ayo chikuru basa iri kukamurwa mapuroteni Kutamiswa zvokudya, kune peptides. Pepsin - ari enzayemu kuti unodokwairira mapuroteni mune imwe acidic ezvinhu. Zvinonzi zvinoberekwa zvinenge zviri mhuka uye nezvinokambaira, vamiririri vekirasi shiri nehove zhinji.

Vakapa enzayemu ndohwaJehovha mapuroteni ari globular mhando, ane samolecular kurema vanenge 34500. Morekuru pachayo achimiririrwa polypeptide cheni inoumbwa mazana matatu namakumi mana amino acids. In wayo sezvamunoita rinewo HPO3 uye nhatu disulfide kubatana hunhu.

Pepsin zvinowanzodavirwa kushandiswa zvokurapa mhosva uye cheesemaking. Mumarabhoritari rinoshandiswa kuti uwane kudzidza puroteni yeimwe, kureva, chikuru mapuroteni mamiriro. Pepsin wechisikigo inhibitor - pepstatin.

A siyana enzayemu

Pepsin ane gumi isoforms. Kusiyana pakati isomers zvose yafanana pepsin electrophoretic injini nemano inaktivatsionnyh ezvinhu, mashoko proteolytic basa. Identifier pepsin - CF 3. 4. 23. 1.

Murume ari mudumbu muto rine mhando manomwe pepsin, uye vashanu vavo zvikuru kuparadzaniswa kugona akawanda:

1. Chokwadi pepsin (A) ane kunonyanyisa basa iri svikiro ph 1.9, uye kuwedzera kusvika 6 inactivated.
2. Pepsin 2 (B) rinoshanda napo svikiro kuti ph = 2.1.
3. Type 3 zvinoratidzwa soro dhigirii basa pane imwe ph = 2.4-2.8.
4. Type 5, hunonziwo gastriksin ane soro dhigirii basa pane imwe ph = 2.8-3.4.
5. Type 7 panguva ph = 3.3-3.9 zviratidzi ane chikuru basa.

Zvichireva enzayemu kugaya

Pepsin iri kusunungurwa gastric mumasero ari deactivated chimiro (pepsinogen), asi basa pacharo enzayemu zvaanopa simba hydrochloric acid. Pasi nawo, ruchapinda muruoko handiyo chimiro. A chinodiwa kuti basa enzayemu pepsin ndiyo Kuvapo acidic kwezvakatipoteredza, ndokusaka inonzi yakapedza pepsin ari duodenum nayo anorasikirwa kwayakaita, sezvo ari ura alkaline kwezvakatipoteredza. Pepsin enzayemu anopindura basa rakakura kugayiwa kirasi yose mhuka, uye kunyanya vanhu. fuma iyi uchiora zvokudya mapuroteni madiki peptide ngetani uye amino acids.

Varume nevakadzi vabereke enzayemu ichi zviratidzi anosiyana. Varume, nokuti kumativi maviri kusvika makumi magiramu pamusoro pepsin paawa, apo mumwe mukadzi - maviri kusvika makumi matatu muzana zvishoma. Masero chikuru, chizvarwa pepsin nzvimbo, zvinonzi voga muchimiro kuputswa pepsinogen. Pashure cleavage of peptides neumwe huwandu N-kuguma pepsinogen achipinda chinoshingaira muchimiro. The tiwedzere iri zvakaitwa nemakemikari anoita hydrochloric acid. Pepsin peptidase uye runovharidzira mamwe ane zvivako uye ndiye anokonzera mapuroteni disaggregation.

Medical mabhizimisi

In mushonga pepsin rinoshandiswa nevakawanda senzira medicament dzimwe hosha chokuita nokusava kugadzirwa enzayemu izvi zvomurwere mudumbu. Kusvina rennet enzayemu pepsin kubva porcine dumbu mucous membranes. The mushonga inowanika muchimiro mapiritsi, Maronda akasiyana yaora neimwe admixture acidin kana Nezvakakuyiwa. Pepsin inhengo uye zvimwe zvinodhaka zvabatanidzwa. Ko ATC Bumbiro A09AA03. Mienzaniso zvokurwara umo pepsinosoderzhaschie mishonga waakaudzwa ndiko chirwere Menetrier raMwari.

Beef pepsin - a ...

Rennet enzayemu pepsin nyama - mumwe inozivikanwa uye kakawanda vaishandisa siyana fuma iyi. Pachezvayo enzayemu anogadzirwa mudumbu rechina mhuru. The chinodhaka akashandiswa pakuburitswa, chakaumbwa makemikari zviviri: pepsin uye chymosin vokuitirwa Proportional zvizhinji. Rennet kushandiswa chizi kugadzirwa, uye ayo chikuru basa - mukaka clot inotungamirwa uye kuita nedanho kuibvisa ose chizi uye chizi zvigadzirwa.

Beef pepsin wobudiswa ura mombe kugadzirwa mishonga kutengeswa, ichipfuura muzvikamu zviviri wokunatswa enzayemu kubva mafuta uye netsvina zviri insoluble. Muitiro nokuita bovine pepsin rinopfuura dzakawanda matanho: dzokubudisa muitiro, salting-panze uye sublimation kuomesa.

Other Applications

The enzayemu pepsin inowedzerwa ichivira. Uyewo rinoshandiswa muna chizi kugadzirwa. Rennet pepsin aiwanzopiwa wokushanda pamwe chymosin kuumba zvakafanana enzayemu kushandiswa hupere mukaka.

Folding muitiro mukaka mapuroteni coagulation kumutumidza, kureva casein chokuumba gel mukaka hwaro. Casein ane chairo mamiriro chidziro, uye peptide chisungo chimwe chete ndiye anokonzera enzymatic mhando pakugwamba mapuroteni. Complex pamwe pepsin uye chymosin pachayo mhosva rotonhorera kubatana pacharo uye inotungamirira coagulation mukaka.

mhedziso

Nokupfupikisa, tinogona kutaura kuti ichi kushingaira chirungiso ndeimwe anokosha makemikari munyaya kugaya zvokudya mudumbu vamiririri makirasi zvipenyu zvakawanda. In kugadzirwa mushonga uye fuma dzinowanzotsigi- kushandiswa senzira mushonga, uye akawedzera kuti rennet kuti kugadzirwa mukaka uye chizi zvigadzirwa.